| Microorganism name |
Komagatella phaffi (Pichia pastoris)
|
| Target proteins |
Ovotransferrin (Mizutani et al., 2014)
|
| Wild-type or GMO |
GMO (Mizutani et al., 2014)
|
| Production mode (intracellular/extracellular) |
Extracellular (Mizutani et al., 2014)
|
| Protein yield (g/L or g/g?) |
0,057 g/L (Mizutani et al., 2014)
|
| Temperature used in study |
29°C (Mizutani et al., 2014)
|
| pH used in study |
pH 6.0 (Mizutani et al., 2014)
|
| C & N source |
Glycerol, yeast nitrogen base (buffered minimal glycerol medium) (Mizutani et al., 2014)
|
| Regulatory status in Europe |
Not allowed |
| Regulatory status in other parts of the world |
No FDA GRAS approval in US, not allowed in Canada |
| Companies |
NA |
| Publications/references |
-
Mizutani, K., Okamoto, I., Fujita, K., Yamamoto, K., & Hirose, M. (2014). Structural and Functional Characterization of Ovotransferrin Produced byPichia pastoris. Bioscience Biotechnology and Biochemistry, 68(2), 376–383. https://doi.org/10.1271/bbb.68.376
-
Spohner, S. C., Müller, H., Quitmann, H., & Czermak, P. (2015). Expression of enzymes for the usage in food and feed industry with Pichia pastoris. Journal of Biotechnology, 202, 118–134. https://doi.org/10.1016/j.jbiotec.2015.01.027
-
Lv, W., & Cai, M. (2025). Advancing Recombinant Protein Expression in Komagataella phaffii: Opportunities and Challenges. FEMS Yeast Research. https://doi.org/10.1093/femsyr/foaf010
-
Pan, Y., Yang, J., Wu, J., Yang, L., & Fang, H. (2022). Current advances of Pichia pastoris as cell factories for production of recombinant proteins. Frontiers in Microbiology, 13. https://doi.org/10.3389/fmicb.2022.1059777
-
Barone, G. D., Emmerstorfer-Augustin, A., Biundo, A., Pisano, I., Coccetti, P., Mapelli, V., & Camattari, A. (2023). Industrial Production of Proteins with Pichia pastoris—Komagataella phaffii. Biomolecules, 13(3), 441. https://doi.org/10.3390/biom13030441
-
Ergün, B. G., Laçın, K., Çaloğlu, B., & Binay, B. (2022). Second generation Pichia pastoris strain and bioprocess designs. Biotechnology for Biofuels and Bioproducts, 15(1). https://doi.org/10.1186/s13068-022-02234-7
|